| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
A Study on Freeze-Drying as a Method of Preserving Mouse Sperm
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| Autores: |
KAWASE, Yosuke
SUZUKI, Hiroshi
鈴木, 宏志
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| Data: |
2011
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| Ano: |
2011
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| Palavras-chave: |
Comet assay
Freeze-dried sperm
ICSI
SCSA
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| Resumo: |
SRD Young Investigator Award
This review describes the study of freeze-dried mouse sperm for practical application in preserving and transporting genetic resources. Freeze-dried sperm can be used to preserve and transport genetic resources; however, there still remain many areas which need to be studied. In particular, it is essential to assure long-term preservation over several decades or centuries. Recently, the theory of accelerated degradation kinetics to freeze-dried mouse sperm has been applied, and found that long-term preservation by conventional methods requires temperatures lower than -80 C. When the relationship between the pressure at primary drying and the preservation potential of freeze-dried mouse sperm was examined, a pressure of 0.37 mbar at primary drying significantly improved the developmental rate to the blastocyst stage. In addition, it has been shown that freeze-dried sperm stored at -80 C with and without transportation can retain their ability to generate viable offspring after storage for up to 2 years. Sperm chromatin structure assay (SCSA) was applied to mouse sperm freeze-dried under several conditions and compared the results with the embryonic developmental rates of freeze-dried sperm after intracytoplasmic sperm injection (ICSI) and with comet assay results. Furthermore, SCSA might be useful for estimation of developmental potential of fertilized eggs derived from ICSI using freeze-dried sperm in mice.
http://www.jstage.jst.go.jp/article/jrd/57/2/57_176/_article/-char/ja/
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/3032
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| Editor: |
日本繁殖生物学会
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| Formato: |
application/pdf
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| Direitos: |
日本繁殖生物学会
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